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DSIP / Pinealon Spray — Alphagenics spray
Cognitive and Neurological Health

DSIP / Pinealon Spray

DSIP + Pinealon · DSIP + Glu-Asp-Arg

Combination of a neuromodulatory nonapeptide and a regulatory tripeptide

Components2
Total10mg
Available presentations
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About DSIP / Pinealon Spray

This spray combines DSIP (delta sleep-inducing peptide), a nonapeptide isolated in 1977 and studied for its possible role in slow-wave sleep and the stress response, with pinealon (Glu-Asp-Arg), a synthetic tripeptide developed in Saint Petersburg and studied in cell and animal models for antioxidant and neuroprotective effects.

The evidence for pinealon is mostly preclinical, and human studies with DSIP have been heterogeneous.

Quick guide · 5 key points

DSIP / Pinealon Spray: two peptides, one blend

This blend combines DSIP, a nonapeptide studied for its link to deep sleep, with pinealon (Glu-Asp-Arg), a tripeptide studied for its antioxidant effect in nerve cells. Here are five things the research shows.

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  1. 1

    Two peptides, two lines of research

    DSIP (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) was isolated in 1977 in rabbits for increasing delta waves and sleep spindles on EEG. Pinealon is a synthetic tripeptide (Glu-Asp-Arg) studied in cells for its antioxidant effect. The blend brings together a sleep line of research and a neuroprotection one.1,2

    DSIPNonapeptide studied for slow-wave sleep and the stress response.
    PinealonTripeptide studied in cells and animals for antioxidant and neuroprotective effects.
    9 + 3amino acids: the DSIP nonapeptide and the pinealon tripeptide
  2. 2

    DSIP in insomnia: from promising to modest

    In early studies in patients with insomnia, DSIP injections before sleep were associated with improved sleep, with sleep structure normalizing after four doses. In a double-blind trial of 16 patients with chronic insomnia, the effects were weak, and the authors judged them unlikely to be of major benefit.3,4

    16chronic insomnia patients in the double-blind trial
  3. 3

    DSIP: a half-life of minutes, yet reaches the brain

    After an intravenous injection, DSIP cleared quickly from plasma: a half-life of about 4 minutes in dogs and 2–3 minutes in a monkey and in rats. In rats, a labeled DSIP analogue has been shown to cross the blood-brain barrier.5,6

    ≈ 2–4 minPlasma half-life in dogs, a monkey and rats
  4. 4

    Pinealon: antioxidant effects in cells and rats

    In cerebellar neurons, neutrophils and PC12 cells under oxidative stress, pinealon was associated with a dose-dependent reduction in reactive oxygen species and in cell death. In the offspring of rats with prenatal hyperhomocysteinemia, giving it to the mothers was associated with better spatial orientation and learning.2,7

    3cell types in which it reduced reactive oxygen species
  5. 5

    The evidence is per component, and limited

    No published study has tested the two peptides together. No DSIP gene or receptor has ever been identified, and a review called its role in sleep poorly documented; in 107 patients, tolerance was good apart from headaches in a few. The evidence for pinealon is cell and animal work, almost all from one Russian group.8,9,10

    Per componentStudies tested each peptide on its own, not the blend
References
  1. Schoenenberger GA, Monnier M. Characterization of a delta-electroencephalogram (-sleep)-inducing peptide. Proc Natl Acad Sci U S A. 1977;74(3):1282-6. PMID 265572
  2. Khavinson V, et al. Pinealon increases cell viability by suppression of free radical levels and activating proliferative processes. Rejuvenation Res. 2011;14(5):535-41. PMID 21978084
  3. Schneider-Helmert D. DSIP in insomnia. Eur Neurol. 1984;23(5):358-63. PMID 6391925
  4. Bes F, et al. Effects of delta sleep-inducing peptide on sleep of chronic insomniac patients. A double-blind study. Neuropsychobiology. 1992;26(4):193-7. PMID 1299794
  5. Kato N, et al. Development of an enzyme immunoassay for delta sleep-inducing peptide (DSIP) and its use in the determination of the metabolic clearance rate of DSIP administered to dogs. Neuroendocrinology. 1984;39(1):39-44. PMID 6379493
  6. Banks WA, et al. Evidence that [125I]N-Tyr-delta sleep-inducing peptide crosses the blood-brain barrier by a non-competitive mechanism. Brain Res. 1984;301(2):201-7. PMID 6547363
  7. Arutjunyan A, et al. Pinealon protects the rat offspring from prenatal hyperhomocysteinemia. Int J Clin Exp Med. 2012;5(2):179-85. PMID 22567179
  8. Kovalzon VM, Strekalova TV. Delta sleep-inducing peptide (DSIP): a still unresolved riddle. J Neurochem. 2006;97(2):303-9. PMID 16539679
  9. Dick P, et al. DSIP in the treatment of withdrawal syndromes from alcohol and opiates. Eur Neurol. 1984;23(5):364-71. PMID 6548969
  10. Khavinson V, et al. EDR Peptide: Possible Mechanism of Gene Expression and Protein Synthesis Regulation Involved in the Pathogenesis of Alzheimer's Disease. Molecules. 2020;26(1):159. PMID 33396470

Components per bottle

Reference amounts of the formulation. The physicochemical data correspond to each individual peptide.

DSIP

W1A2G3G4D5A6S7G8E9
Molecular formula
C35H48N10O15
Molecular weight
848.8 g/mol
CAS number
62568-57-4
PubChem CID
68816
Length
9 aa

Pinealón

GluAspArg
Molecular formula
C15H26N6O8
Molecular weight
418.4 g/mol
CAS number
175175-23-2
PubChem CID
10273502
Length
3 aa

Five findings from studies

Summary of results reported in the scientific literature. Each finding describes what was observed in the cited study, in its specific population and conditions; it does not constitute a therapeutic indication.

  1. Preclinical

    DSIP: in the original studies in rabbits, isolated DSIP has shown an increase in slow-wave (delta) electroencephalographic activity and in sleep spindles.

    Schoenenberger GA, Monnier M. Proc Natl Acad Sci U S A. 1977;74:1282-1286 · PMID 265572
  2. Clinical study

    DSIP: in patients with insomnia, administration of DSIP has been associated with improved sleep, with a normalization of its structure after several administrations.

    Schneider-Helmert D. Eur Neurol. 1984;23:358-363 · PMID 6391925
  3. Preclinical

    DSIP: in rats subjected to hypoxia, pretreatment with DSIP has been associated with preservation of the respiratory activity of brain mitochondria.

    Khvatova EM et al. Peptides. 2003;24:307-311 · PMID 12668217
  4. In vitro

    Pinealon: in cerebellar granule cells, neutrophils and PC12 cells under oxidative stress, it has shown a dose-dependent reduction in reactive oxygen species and in necrotic cell death.

    Khavinson V et al. Rejuvenation Res. 2011;14:535-541 · PMID 21978084
  5. Preclinical

    Pinealon: in the offspring of rats with prenatal hyperhomocysteinemia, giving it to the mothers has been associated with better spatial orientation and learning, and with cerebellar neurons more resistant to oxidative stress.

    Arutjunyan A et al. Int J Clin Exp Med. 2012;5:179-185 · PMID 22567179

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